Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENKIIYFLCTGNSCRSQMAEGWAKQYLGDEWKVYSAGIEAHGLNPNAVKAMKEVGIDISNQTSDIIDSDILNNADLVVTLCGDAADKCPMTPPHVKREHWGFDDPARAQGTEEEKWAFFQRVRDEIGNRLKEFAETGK 1JL3 Chain:D ((4-139)) ---KIIYFLCTGNSCRSQMAEGWAKQYLGDEWKVYSAGIEAHGLNPNAVKAMKEVGIDISNQTSDIIDSDILNNADLVVTLCG------------VKREHWGFDDPARAQGTEEEKWAFFQRVRDEIGNRLKEFAETGK

General information: TITO was launched using: RESULT: Template: 1JL3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 615 -67115 -109.13 -541.25 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain D : 0.96 3D Compatibility (PKB) : -109.13 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.675

(partial model without unconserved sides chains): PDB file : Tito_1JL3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JL3-query.scw PDB file : Tito_Scwrl_1JL3.pdb: