Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLVIVIHPNLETSVVNKTWMNRLKQEKDITVHDLYGEYPNFIIDVEKEQQLLLDHERIVFQFPMYWYSSPALLKQWEDDVLTHGWAYGTGGTKLHGKELLLAISLGAQESDYQAGGEYNITISELIRPFQVTANYIGMRFLPAFTQYGTLHLSKEDVKNSA----ERLVDYLKAEH 3L9X Chain:B ((240-411)) ---LIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNIDIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLWAVTTGGGESHFEIGAHPGFDV--LSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQARHYKQRLLEWQEAH-

General information: TITO was launched using: RESULT: Template: 3L9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 750 -122162 -162.88 -727.15 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -162.88 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.614

(partial model without unconserved sides chains): PDB file : Tito_3L9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L9X-query.scw PDB file : Tito_Scwrl_3L9X.pdb: