Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSDQKFIILDKSHEVGESWKDR--YDSLVL-FTSRMYSSL---PGMHLEGEKHGFPSKNEIVAY-----LKKYVKKFEIPIQLRTEVISVLKIKNYFLIKTNREEYQTKNLVIATGPFHTPNIPSISKDLSDNINQLHSSQYKNSKQLAYGNVLVVGGGNSGAQIAVELSK-ERVTYLACSNKLVYFPLMIGKRSIFWWFDKLGVLHASHTSIVGKFIQKKGDPVFGHELKHAIKQKEIILK--KRVIAAKQ--NEIIFKDSSTLEVNNIIWATGFRNPLCWINI-K-GVLDQEGRIIHH-RGVSPVEGLYFIGLPWQHKRGSALLQGVGNDAEYIVKQMNGE 4YWO Chain:A ((15-330)) MHKLAIIGYGAAGFAAMIKANELGVKPVLIGKGE-IGGTCVNVGCVPSKRMLYIAEIYKKAREVTGSEVYPPFSSFQEKDGLVQEMRKTKYEDLLSYYDVELIQG-EARFISPH----AVKVNGQVIEAEKFVIATGS--SPLIPRIPGLDKV--GFWTNREALSP-DRRIDSLAVIGGRALALEFAQMYSRMKVEVAILQRSP-VLIPDW--EPEASV------------------------------EARRIMENDGVAVVTGVNVKEVRKGAGKIVITDKGEVEADEILLATGRKPNVDLGLENAGVRLNERGGIKVDDELRTDNPHIYAAGDVLGG---KMLEALAGRQGSIATENALT-

General information: TITO was launched using: RESULT: Template: 4YWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1578 -2566 -1.63 -8.64 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -1.63 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_4YWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YWO-query.scw PDB file : Tito_Scwrl_4YWO.pdb: