Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQ-----TEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKE----GIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEE--A-LPAGEQFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWL-NKENIAHISDEALRSGLVKAAWPGRLELVQEHPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDF---PRASLAKDLY----D-ASEISNKSWSEDPDDVIKFIESKK-----------GSNEIVLITGSLYFISDIRKRLK 2VOS Chain:A ((32-483)) ---------HLLDQRW--RIDPSLTRISALMDLLGSPQRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQAS---PAMSKFEVLTAMAFAAFADA-PVDVAVVEVGMGGRWDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPSPDTVAVIGRQVPKVMEVLLAESV--RADASVARED-SEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGA----QLDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDSVVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDV----ATSLVDDAAADPDVAG--RTGIVITGSVVTAGAARTLFG

General information: TITO was launched using: RESULT: Template: 2VOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2352 -103815 -44.14 -258.25 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -44.14 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_2VOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VOS-query.scw PDB file : Tito_Scwrl_2VOS.pdb: