Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNTELELLRQKADELNLQILKLINERGNVVKEIGKAKEAQGVNRFDPVRERTMLNNIIENNDGPFENSTIQHIFKEIFKAGLELQEEDHSKALLVSRKKKPEDTIVDIKGEKIGDGQQRFIVGPCAVESYEQVAEVAAAAKKQGIKILRGGAFKPRTSPYDFQGLGVEGLQILKRVADEFDLAVISEIVTPAHIEEALDYIDVIQIGARNMQNFELLKAAGAVKKPVLLKRGLAATISEFINAAEYIMSQGNDQIILCERGIRTYETATRNTLDISAVPILKQETHLPVFVDVTHSTGRRDLLLPTAKAALAIGADGVMAEVHPDPSVALSDSAQQMAIPEFEKWLNELKPMVKVNA 3TFC Chain:B ((29-374)) ----NLEELRTQVDQLNIDLLELISKRANLVQ------------------EREMLNTILAANEGPFEDSTVQKLFKEIFKAGL------HSKALLVSRKNKKEDTIVTVKGLPIGNGEPVFVFGPCSVESYEQVAAVAESIKAKGLKLIRGGAFKPRTSPYDFQGLGLEGLKILKRVSDEYGLGVISEIVTPADIEVALDYVDVIQIGARNMQNFELLKAAGRVDKPILLKRGLSATIEEFIGAAEYIMSQGNGKIILCERGIRTYEKATRNTLDISAVPILKKETHLPVMVDVTHSTGRKDLLLPCAKAALAIEADGVMAEVHPDPAVALSDSAQQMDIPEFEEFWNAI--------

General information: TITO was launched using: RESULT: Template: 3TFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1616 -173479 -107.35 -538.75 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -107.35 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_3TFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TFC-query.scw PDB file : Tito_Scwrl_3TFC.pdb: