Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMTSRKLSDILKQRLQHENRSFLFDREKDTLRVEDQTTKKGITLDLPPIIAKWELKKDEAIDEIVYYVSEAMTAMEGKAQEMT-----------GKETRIYPVIRSTSFPDKSSEDIPL-----IYDDHTAETRIYY-------ALDLG------KTYRLIDQRMLEKENWTKERIRETAAFNLRSLPTVVKEDTVAGNYFYFFRANDGYDASRILNEAILNEYKQHAEGELAISVPHQDVLILADIRNESGYDILGQMSMSFFAGGTVPITALSFLYNEGKLEPVFILAKSRPKKD 1QW9 Chain:A ((351-458)) ---------------------------------------------------------------QLVNVIAPIMTEKNGPAWKQTIYYPFMHASVYGRGVALHPVISSPKYDSKDFTDVPYLESIAVYNEEKEEVTIFAVNRDMEDALLLECDVRSFEDYRVIEHIVLEHDN-------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1QW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 201 6262 31.15 79.27 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 31.15 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.120

(partial model without unconserved sides chains): PDB file : Tito_1QW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QW9-query.scw PDB file : Tito_Scwrl_1QW9.pdb: