Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILFAVSECTPFVKSGGLADVAGALPKALARLGNEVAVMLPKYSQIPEPWKKRMKKQAEC---TVAVGWRQQYCGIEHMAENDVNYYFIDNEYYFNRDSLYGHY---------DDGERFAFFSRAV--LEAAKVVNVQADIVHTHDWHTAMVNYLLKEEYRKHPFYERMKSVLTIHNLQFQGIFPPDVTHDLLGLEMDHFHYERLECNGFVNFMKAGIIAADHVTTVSPTYRNEIMTPYYGEQLEQVLQYREDDVTGILNGIDDTFYQPKSDPYIEAQYDSGDLACKLENKTKLQQRMGLPEKNDIPLISMVTRLTKQKGLDLVRRIMHELLEEQDIQLVVLGTGEREFEDYFRYAEFAFHEKCRAYIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRETGGLYDTV-----RAYQEEEGTGNGFTFSAFNAHDLKFTIERALSFYCQQDVWKSIVKTAMNADYSWGKSAKEYQRIFEQVTRSGRDVLE 1RZU Chain:B ((1-478)) MNVLSVSSEIYPLIKTGGLADVVGALPIALEAHGVRTRTLIPGYPAV----KAAVTDPVKCFEFTDLLGEKADLLEVQH---ERLDLLILDAPAYYERSG--GPYLGQTGKDYPDNWKRFAALSLAAARIGAGVLPGWRPDMVHAHDWQAAMTPVYMR--YAETP---EIPSLLTIHNIAFQGQFGANIFSKL-ALPAHAFGMEGIEYYNDVSFLKGGLQTATALSTVSPSYAEEILTAEFGMGLEGVIGSRAHVLHGIVNGIDADVWNPATDHLIHDNYSAANLKNRALNKKAVAEHFRI-DDDGSPLFCVISRLTWQKGIDLMAEAVDEIVSLGG-RLVVLGAGDVALEGALLAAASRHHGRVGVAIGYNEPLSHLMQAGCDAIIIPSRFEPCGLTQLYALRYGCIPVVARTGGLADTVIDANHAALASKAAT--GVQFSPVTLDGLKQAIRRTVRYYHDPKLWTQMQKLGMKSDVSWEKSAGLYAALYSQLISK------

General information: TITO was launched using: RESULT: Template: 1RZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2738 -60780 -22.20 -132.42 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -22.20 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_1RZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RZU-query.scw PDB file : Tito_Scwrl_1RZU.pdb: