Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQSKQLVRLIEIAIMTAAAVILDIVSGMFLSMP-QGGSVSIMMIPIFLISFRWGVKAGLTTGLLTGLVQIAIGNLFAQHPVQLLLDYIVAFAAIGI-SGCFASSVRKAAVSKTKGKLIVSVVSAVFIGSLLRYAAHVISGAVFFGSFAPKGTPVWIYSLTYNATYMVPSFIICAIVLCLLFMTAPRLLKSDKA 4HZU Chain:S ((1-164)) -MTKGSLKENTIAAVLIAMTVALSILVVI--PIPATKGIVTLCEVGIYTSAILYGRRMGLLVGGASGFLIDILTGYPVWC-----LFSLVIHGTQGLVVGWLLPRHHKG---------IRSMLLPLLVGSLVMVIGYCLATTLLFG------WP----AGLASIFGNVVQVGFGAGVTLSIVGPLTRLKPD---

General information: TITO was launched using: RESULT: Template: 4HZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 730 -151330 -207.30 -934.13 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain S : 0.66 3D Compatibility (PKB) : -207.30 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_4HZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HZU-query.scw PDB file : Tito_Scwrl_4HZU.pdb: