Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFTYYNPTKLIFGKGQLEQLRKEFKRYG-KNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDIFSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYELRETILYAGTIALNGTLQMGYF-GDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIAD----IAAKEMEHGG--FGNFQKLNKDDVLAILRASL 1VLJ Chain:B ((13-403)) MENFVFHNPTKIVFGRGTIPKIGEEIKNAGIRKVLFLYGGGSIKKNGVYDQVVDSLKKHGIEWVEVSGVKPNPVLSKVHEAVEVAKKEKVEAVLGVGGGSVVDSAKAVAAGALYEGDIWDAFIGKYQIEKALPIFDVLTISATGTEMNGNAVITNEKTKEKYGVSSKALYPKVSIIDPSVQFTLPKEQTVYGAVDAISHILEYYFDG-SSPEISNEIAEGTIRTIMKMTERLIEKPDDYEARANLAWSATIALNGTMAVGRRGGEWACHRIEHSLSALYDIAHGAGLAIVFPAWMKYVYRKNPAQFERFAKKIFGFEGEG---EELILKGIEAFKNWLKKVGAPVSLKDAGIPEEDIDKIVDNVMLLVEKNLKPKGASLGRIMVLEREDVREILK---

General information: TITO was launched using: RESULT: Template: 1VLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2279 -21229 -9.32 -55.43 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -9.32 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_1VLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VLJ-query.scw PDB file : Tito_Scwrl_1VLJ.pdb: