Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEYGFKDDSLSLHTDLYQINMAETYWRDGIHEKKAIFELFFRRLPFENGYAVFAGLEKAI-EYLENFKFTDSDLSYLQDE-------LGYHEDFIEYLR-GL--SFTGSLYSMKEGELVFNNEPIMRVEAPLVEAQ----LIETALLNIVNYQTLIATKAARIKGVIG-------------DEVALEFGTRRAHEMDAAMWGARAALIGGFSATSNVRA----GKRFNI--PVSG----THAHALVQAYR--DEYTAFKKYAETH-KDCV-FLVDTYDTLRSGMPNAIRV-AKEFG-DR--INFIGIRLDSGDLAYLSKKARKMLDE-AGF-----------TDAKVIASSDLDEHTIMNLKA----QGA--RIDVWGVGTKLITAYDQPALGAVYKLVAIEEDGKMVDTIKISS-NPEKVTTPGRKKVYRIINQSNHHSEGDYIALYDEQVNDQKRLRMFHPVHTFISKFVTNFYAKDLHELIFEKGILCYQNPEISDIQQYVQDNLSLLWEEYKRISKPEEYPVDLSEDCWSNKMQRIHEVKSRIEEELEEE 4LVF Chain:A ((8-486)) -------EFNILLATDSYKVTHYKQYPPNTSK---VYSYFECREK--KYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQ-SWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGA-SAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYN-ACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPA-------GNFVTLEEGKGD------------------LE-EYGQDLLHTVFKNGKVTKS-YSFDEIRKNAQLNIEL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2093 51427 24.57 127.29 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 24.57 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_4LVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LVF-query.scw PDB file : Tito_Scwrl_4LVF.pdb: