Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNQGSPQLVSLVDPYVYQTIKKLIGSRFVIQTVRDT-VRGRLIDVKP-DHITIEGAR----------NSVCLIRIQHMISVTPDYSERV 4F7U Chain:F ((8-76)) -----------------KPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVLYIRGVE----

General information: TITO was launched using: RESULT: Template: 4F7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 184 -5099 -27.71 -89.46 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain F : 0.61 3D Compatibility (PKB) : -27.71 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.658

(partial model without unconserved sides chains): PDB file : Tito_4F7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F7U-query.scw PDB file : Tito_Scwrl_4F7U.pdb: