Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLNMIRRLLMTCLFLLAFGTTFLSVSGIEAKDLSKWVQEHQEKHLKHAGLRLKALQQKQTQTTSAAEDKTKPLEEAFDWDEYPVQRVTATGYTAGAESTGKNPGDPLYGLTYSGVKVKRDLYSTVAADPSVFPIGTILFIPNY------------GLGVVADTGSAIKG-NRLDLYFETVKDVYNEWGK--KTLDVYVIKKGTGKITEDELEKLNETKSLQVFRNQYKTVKE 2PNW Chain:A ((272-365)) ------------------------------------------------------------------------------------------------------------GPIAAAKVPLVAG--RALAVDRLIHTFGLPFFIHAPTLTHLDDGKPFARLMLALDTGSAIVGPARGDIFTGSGFEAGELAGTVRNEADFYILLPRI-----------------------------

General information: TITO was launched using: RESULT: Template: 2PNW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 281 -16188 -57.61 -204.91 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -57.61 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_2PNW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PNW-query.scw PDB file : Tito_Scwrl_2PNW.pdb: