Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQQNQKISNPQTPVPTTSEMNDRDFVNELLTTEKYMTTAYCTALHEFSH---ESLYQDIQSIFDESQKAQRKLYDLMFQYGWYSVEAEDSQKLQQSYQKFQQTIQQQSPYQQ 3FVB Chain:A ((20-87)) --------------GSMKGEPKVIERLNEALFLELGAVNQYWLHYRLLNDWGYTRLAKKEREESIEEMHHADKLIDRIIFLE-------------------------------

General information: TITO was launched using: RESULT: Template: 3FVB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 134 -3683 -27.48 -56.65 target 2D structure prediction score : 0.88 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -27.48 2D Compatibility (Sec. Struct. Predict.) : 0.88 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_3FVB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FVB-query.scw PDB file : Tito_Scwrl_3FVB.pdb: