Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTEL---RRLIPDVL-FLIAVGNNSVRKQLAERLGLGKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQADARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQEGAHVGTGASVIPQIIIGAWSIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG 4M99 Chain:C ((5-206)) -RKLAVIGAGGHGKVVAELAAALGTYGEIVFLDDRTQ------GSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITENAAALGFKLPVLIHPDATVSPSAIIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHDCLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDGMTVAGNPAK-----------

General information: TITO was launched using: RESULT: Template: 4M99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1213 -30957 -25.52 -156.35 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -25.52 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4M99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M99-query.scw PDB file : Tito_Scwrl_4M99.pdb: