Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVQQKVEVRLKTGLQARPAALFVQEANRFTSDVFLEKDGKKVNAKSIMGLMSLAVSTGTEVTLIAQGEDEQEALEKLAAYVQEEV 1MU4 Chain:B ((1-85)) MVQQKVEVRLKTGLQARPAALFVQEANRFTSDVFLEKDGKKVNAKSIMGLMSLAVSTGTEVTLIAQGEDEQEALEKLAAYVQEEV

General information: TITO was launched using: RESULT: Template: 1MU4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 290 -33702 -116.21 -396.49 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 1.00 3D Compatibility (PKB) : -116.21 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.729

(partial model without unconserved sides chains): PDB file : Tito_1MU4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MU4-query.scw PDB file : Tito_Scwrl_1MU4.pdb: