Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNRNQAIIASLCYFSVFIAPIIVPIV----------AYFVVNEKETKRHAIRSLISHIVPFVGWL--------FLFIALLGGAVAIDGDSLLPV---FVIIGGAVIYFL-----VVIGIIIWNVIQGIKVLRAA 4CHD Chain:A ((547-681)) DPFSRAKSLLKSTILHAERCKEFVGNMLEEYQDPAETTVQSLVPINTWGKSAKRKLQEEITSDPDWHQCPRKRAKMSYLAIIAGSIQDRDKKQTNVPRAFMLRGSQIEYDMKATRGLVVDTTNRIIVGGETVLRE-

General information: TITO was launched using: RESULT: Template: 4CHD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 414 -27037 -65.31 -252.68 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -65.31 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_4CHD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CHD-query.scw PDB file : Tito_Scwrl_4CHD.pdb: