Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVLSRKINEAIQIGADIEVKVIAVEGDQVKLGIDAPKHIDIHRKEIYLTIQEENNRAAALSSDVISALSSQKK 2MFH Chain:C ((1-52)) MLILTRKVGESINIGDDITITILGVSGQQVRIGINAPKDVAVHREEIYQRIQ----------------------

General information: TITO was launched using: RESULT: Template: 2MFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 104 -18833 -181.09 -362.17 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -181.09 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.340

(partial model without unconserved sides chains): PDB file : Tito_2MFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MFH-query.scw PDB file : Tito_Scwrl_2MFH.pdb: