Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFARDIGIDLGTANVLIHVKGKGIVLNEPSVVALDKNSGK--VLAVGEEARRMVGRTPGNIVAIRPLKDGVIADFEVTEAMLKHFINKLNVKGLFSKPRMLICCPTNITSVEQKAIKEAAEKSGGKHVYLEEEPKVAAIGAGMEIFQPSGNMVVDIGGGTTDIAVISMGDIVTSSSIKMAGDKFDMEILNYIKREYKLLIGERTAEDIKIKVATVFPDARHEEISI--RGRDMVSGLPRTITVNSKEVEEALRESVAVIVQAAKQVLERTPPELSADIIDRGVIITGGGALLNGLDQLLAEELKVPVLVAENPMDCVAIGTGVMLDNMDKLPKRKLS 4CZJ Chain:B ((5-327)) ----DIAIDLGTANTLIYQKGKGIVLNEPSVVALRNVGGRKVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHN------PKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAPADGEGLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH----------

General information: TITO was launched using: RESULT: Template: 4CZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1867 -3150 -1.69 -10.06 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -1.69 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_4CZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZJ-query.scw PDB file : Tito_Scwrl_4CZJ.pdb: