Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHI-----SESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2B8T Chain:B ((11-200)) ------IGWIEFITGPMFAGKTAELIRRLHRLEYADVKYLVFKPKI----------------LPSVEVESAPEILNYIMSNSFNDETKVIGIDEVQFFDDRICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFTYADKITKLTAICNECGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPNR---

General information: TITO was launched using: RESULT: Template: 2B8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 835 -23042 -27.59 -135.54 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -27.59 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2B8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B8T-query.scw PDB file : Tito_Scwrl_2B8T.pdb: