Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKVSVIMTSYNKSD-YVAKSISSILSQTFSD--FELFIMDDNSNEETLNVIRPFLNDNRVRFYQSD-ISGVKERTEKTRYAALINQAIEMAEGEYITYATDDNIYMPDRLLKMVRELDTHPEKAVIYSASKTYHLNENRDIVK--------ETVRP---------AA-QVT----W---NAPCAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHFYP-FYPLDEELDLNYITDQSIHFQLFELEKNEFVRNLPPQRNCRELRESLKKLGMG 5AJO Chain:A ((135-363)) LPATSVVITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYSNDPEDGALLGKI--EKVRVLRNDRREG-------L-MRS-RVRGADAAQAKVLTFLDSHCECNEHWLEPLLERVAED-RTRVVSPII--DVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEEL----GKYDMMM-DVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRK--------------------------------------

General information: TITO was launched using: RESULT: Template: 5AJO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 897 -9215 -10.27 -46.30 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -10.27 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_5AJO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AJO-query.scw PDB file : Tito_Scwrl_5AJO.pdb: