Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKIKDDCIELELTPRR-YQELDDDPFILSVFELLE------NKKAVVRDFSAVLLESEYKVLISGIETMIKGNQDSISLETIEPFLFLSIDQENGNYRIKI-KIIFDDYKESKSNSNLFEI-----NCNEEKLESFVT-ALKLNLE---------------NTKNPSKLP 4XUU Chain:A ((1-169)) --SRSHQELISQLLQSYMKLLLPDDEKFHGGWALIDCDPSLIDATHRDVDVLLLLSNSAYYVAYYDDEVDKVNQYQRLSLENLEKIEIGPEPTLFGKPKFSCMRLHYRYKEASGYFHTLRAVMRNPEEDGKDTLQCIAEMLQITKQAMGSDLPIIEKKLEAKASKPHEDII

General information: TITO was launched using: RESULT: Template: 4XUU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 499 46372 92.93 331.23 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 92.93 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.287

(partial model without unconserved sides chains): PDB file : Tito_4XUU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XUU-query.scw PDB file : Tito_Scwrl_4XUU.pdb: