Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQACIAIIIILLTVAAYLAMVKLYKRFPLPFLIPVLTTTILIVAALMMFHVSYEGYMIGGKWINSLLGPAVVALAYPLYKQWHIIVKHCVPILGGVLVGLCMGMISGLIFAEAFGIDHDLLLSILP----KSITTPVAIQIAAGLGGVPSMTVVFVMIAGFSGVILGPLFLKWLRIRSSLGQGIALGSASHALGTSKALEYGELAVSMSSVSMTLCAVLGSFFGPLVVWLFHI 3ZUX Chain:A ((218-313)) --------------------------------------------------------------------------------ASKGKIMESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKALTIEVGMQNSGLAAALAAA-HFAAA-PVVAVPGALFSVWHNISGSLLATYWAAK--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 271 -44265 -163.34 -481.14 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -163.34 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_3ZUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZUX-query.scw PDB file : Tito_Scwrl_3ZUX.pdb: