Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKKRVSMFLVAL--TMCGGLFVT-PAKAVKTTNYAEEIAALQPGTTPEEIMKSASQIAKQQHVKQDVILKQFYKEITADKAEG-DRLAKESGMSI-MGGSSGTKKLPTSA-KGNIYYTNS-YTAYYNHGHVGMYSAADK--IVESVPSDGVRQIAYNARDVEDNSIVQTVSVSSSQK------TAAADWAVSKVG-DPYSFNFVNNRNTGHDGAKNCSKLLWSAFLLKAGIDIDSNGGLGVYPRDITSSSYTTTIMTIY- 1ZS9 Chain:A ((4-257)) LSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLDGAVPIPAASGNGVDDLQQMIQAVVDNVCWQMSLDKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREASAAEEAD---VHVAVVV------RPGNAGLTDDEKTYYSLITSFSELYL

General information: TITO was launched using: RESULT: Template: 1ZS9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 -1143 -1.06 -4.84 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -1.06 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_1ZS9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZS9-query.scw PDB file : Tito_Scwrl_1ZS9.pdb: