Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESHIYRIIKNKLTIIIFTIIILIPCVDISLLLMTNTEYHPAYAFFLSGTSVGHASQMILLWFLPLYFLLLCADDSIQDYKTGYHYILISKVG----RKKYCLEKIFTSFIISFLTMFLSLILNFLLVQVFFFKGTFK--NDLDQIKFPDNSLYTFSMAHPYIAIVLFSIICCIMS-GFVGALGSSLSLLFRDKKYAYPASFFIWFVLILKNKSLMFLFQPFTEYGYNVLLPILCLSIFIFLIIISSIVLYEAKYNEN 3EUH Chain:C ((10-215)) MPVKLAQALANPL----------FPALDSALR----SGRHIGLDELDNHAFLMDFQEYLEEFYARYNVELIRAPE-------GFFYLRPRSTTLIPRSVLSELDMMVGKILCYLYLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLNRLRRLGMVWFMGHD------SSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIEN-------------------------

General information: TITO was launched using: RESULT: Template: 3EUH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 798 -67606 -84.72 -339.73 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain C : 0.57 3D Compatibility (PKB) : -84.72 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_3EUH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EUH-query.scw PDB file : Tito_Scwrl_3EUH.pdb: