Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQIMIFLTSFMLLAMTGQTALAKDVQVSGSLLGKSSQEQAKQQVLTSELITLYGSKDSAELTYQIPAGASSGNQQLVIEYEASNLLISPSSLTVVIDDEPVKTLKLDGDSKRKTVKLNLNKSQSAQGYHNVSLKFYGVMKEGVCVRQDTSGNWIKIYPDSRLTLADSSEAKGTSLDHYPYPFAQSGNTAEETAIVIPDNPSSAEIEAAVKTEGYLKTVDSSVSIAYVTESELKKIDKPTIVIGVDKHWNGKVKKLLKQAGLQAKGENLLLAERVLKAEGKQQPVLFAQAASEDALTKKISVMTDQTYTGQLSGDTLSISKLQQTEKKESDKLTLENFGAGDITIGADKTSSAHYFY-PASAVLDENQSAKLSLKLKKSETIQASTAENESASQAAELKVMINGQPHSVRLDELGKEDKNGFYHVTVKVDPKLLQKN-RYIDIQFVTTGLKENNPCNTTDEEKWVFIDKNSTL----SYAIKGMSPSADFQEWPLPYAG----NQDQTTLIVLP-----------DTVSQSKLEELSLVTESFGNEAQHSYTVKKSSDVTANDAKGRNLIFIGGINQFSLLK---EKSSDLLVPQEKNGSFDVSSFEM------LNETTKQVVFTQASVWDSRYTMAVFAPSKGDGTAVTKEIISYLNSNDE---SATVLNETNSQQVFTNHQQL--KSETNSSDAEQPTQDHSQKWMYIGVLALIMVVAAVFIWIAVRRKKRKTDTE 4HG6 Chain:B ((36-702)) --PWIIPLRPLAETAQVG------------------------------PLFRLQGQQARAAFRLFLPTEAVGGTLTLAQRSSID-ILPESSQIIVRMNDQEIGRFTPRQFGALGAVTMPLGEAVRA-GDNLVTIEAQH-RHRIYCGADAEFDLWTEVDLSQSGVALPAAAIGTEPTSFIAALTAQAES-GRPVEIRTPTPPDEATLRTLAQALGRPLPDEALPLALSKPWSAETGPTYARITLLPSD------------------------ADRVSIRRGGDGAVVLVLEHPPEGSP-------NASLVADLLGATPTLPPPTLPQIPPGRVVTLADMGVDTILTDNRYFNRDIDFQLPDDWLLLASQKAQIGIDYGFAG----------GLPEGALLLVKVNG--TTVRMLPLDRDAAPVKPRLDIRFPARLLHPGPNRLSFESVIPGNPPDQPCPAS-AGDLMQVLSSTDLEVPPSPRMQMADMARDLAQVTPASVHPATPDGLARTLPFMAAFREVPDAAPVDLTVAGLHDIATVPLNEEGLTPRLLALTLLPSTGPPANALAPLGAAPGEGVMPPLVESNWSDRAQTFVQATLQPVIQTVRRMLRPGDGNLAEWLATRKGTAMLLAPEPGKLWVILGPEAEPARVAEALAMAPRSPGGPRGQVAVLGSDGRWSSWSKPGLLPELREPVSLDNVRSVVGNVASARPPLLLGGMLGLAWISAAIAVGFVLRTR---

General information: TITO was launched using: RESULT: Template: 4HG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3238 -129176 -39.89 -208.35 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -39.89 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_4HG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HG6-query.scw PDB file : Tito_Scwrl_4HG6.pdb: