Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFAFKLYEYNVWANQQIFNRLKELPKEIYHQEIQSVFPSISHVLSHVYLSDLGWIEVF-SGKTLSDALAL---AEQLKEQTEAKEIEEMEDLFLRLSERYILFLQQ--KEQL---NKPLQIQNPSSGIMKTTVSELLPHV---VNHGTYHRGNITAMLRQAGYASAPTDYGLYLFMTKTEKA 2QNL Chain:A ((10-162)) ----VRLALDAWNTQSSRTDKLIQSLSNEALAVETAPGRNSGTYLLGHLTAVHDAMLPLLELGDTLYPQLAPVFIQNPDKSGLEKPEINDLRLYWSLVQERLANQFNQLQPADWFNKHAAISR--EDF--LKEPHRNKLSVLINRTNHMAYHLGQLAYLKK-----------------------

General information: TITO was launched using: RESULT: Template: 2QNL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 487 -56022 -115.03 -397.32 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -115.03 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_2QNL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QNL-query.scw PDB file : Tito_Scwrl_2QNL.pdb: