Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQTILSNGIAMVLIILIINIVYVSFFTIRMILTLKGQRYLAAGISTIEILVYVTGLSLVLDNLDQIQNVIAYALGYGLGVIVGMKIEEKLALGYIMVNVITKE-LDLDLPKQLREKG-YGVTNWVAGGLEG-----------------DRTA-LQILTPRRYELQLYDTIKTLDS-----KAFIIAYEPKTIHGGFWVKAVKKRRIKE 1VFJ Chain:A ((4-102)) -------------------------------------------------------------------------------------------------IVAIVRPEKLNEVLKALFQAEVRGLTLSRVQGHGGETERVETYRGTTVKMELHEKVRLEIGVSEPFVKPTVEAILKAARTGEVGDGKIFVLPVEKVYRI-------------

General information: TITO was launched using: RESULT: Template: 1VFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 -36901 -138.21 -498.66 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -138.21 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_1VFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VFJ-query.scw PDB file : Tito_Scwrl_1VFJ.pdb: