Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSIDSAIKKAADKYGVDEKLIRAVIKQESGFNAKAVSGAGAMGLMQLMPSTA-----SSLGVSNP------LDPQQNVEGGTKYLKQMLDKYDGNVS-------MALAAYNAGPGNVDRYGGIPPFQETQNYVKKITSVYYA 4CFO Chain:B ((178-290)) ---------------------------------------------------------------VRQASRKYGVDESLILAIMQTQSSFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAMLNNVYLGGIDNPTSRRYAVITAYNGGAGSVLR-----------------------

General information: TITO was launched using: RESULT: Template: 4CFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 357 -45872 -128.49 -482.86 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -128.49 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_4CFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CFO-query.scw PDB file : Tito_Scwrl_4CFO.pdb: