Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLAGGINPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCGALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPAKHKKPFILDMATSKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLSTFGGP---KGYGLSIVVDVFSGLLAGAAFGPHIAKMYN-GLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAVGFERVYVPGEIEQLHEERNKKNGISIARSVYEFLKSR 1Z2I Chain:D ((9-335)) --TVLARLDELERFCRAVFLAVGTDEETADAATRAMMHGTRLGVDSHGVRLLAHYVTALEGGRLNRRPQISRVSGFGAVETIDADHAHGARATYAAMENAMALAEKFGIGAVAIRNSSHFGPAGAYALEAARQGYIGLAFCNSDSFVRLHDGAMRFHGTNPIAVGVPAADDMPWLLDMATSAVPYNRVLLYRSLGQQLPQGVASDGDGVDTRDPNAVEMLAPVGGEFGFKGAALAGVVEIFSAVLTGMRLSFDLAPMGGPDFSTPRGLGAFVLALKPEAFLERDVFDESMKRYLEVLRGS-PAREDCKVMAPGDREWAVAAKREREGAPV-----------

General information: TITO was launched using: RESULT: Template: 1Z2I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1864 -132294 -70.97 -409.58 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -70.97 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_1Z2I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z2I-query.scw PDB file : Tito_Scwrl_1Z2I.pdb: