Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWSNLQRQQLYTESDAKLRMPKAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVIDATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVIPG-ETPCLSCLFEQIPVGGATCDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNNSHMKINVNHVRREE-CPSCGANAVYPYLQDWNTPKAAVLCGRDTVQVRSESLKRIPKQELIKRLKTIGKVEANAFLLHIFYEDFRIVIFNDGRALVHGTNDVKEANSVLARVIGL 1ZKM Chain:C ((10-245)) ---RYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADDDDVHLSNLQRQILFTTED--IDRPKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSG----IETPAGELRLFDGKSSQWRSLALRRASGCPVC------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZKM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1342 -158368 -118.01 -676.78 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -118.01 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1ZKM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZKM-query.scw PDB file : Tito_Scwrl_1ZKM.pdb: