Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIFAHRGASGQFPENTMLAFEKGIEAGADGIELDVQLTKDGRIVVIHDERLNRTTSLKGFVKDTAYDEVKTANAAAGHDQAYSD--------IKVPLLEDVLSWAVKKDFLINIELKNSVIRYEGMEEKVLEAVKRFNV-EDRVILSTFNHDSLALCARLAPHI------------------------ERAVLTSDVLYQADRYIASIPASGYHPKI---NSPGVTDEVLKKM--RNGLIKVRPYTVNRPEDMKRLIEAGADGMFTDFPEKASALLKNE 4R7O Chain:G ((20-290)) -----AHRGASGHAPEHTFASYDLVKKMKADYLELDIQLTKDGQLIAMHDTAVDRTTNGTGEVRDKTLSEIKSLDAGSWFNKAYPEKAKQEYVGQKVPTLEEIFQ-KYGRSMKYYIETKSPDV-YPGMEEKLLALLEKYNLI--RVMIQSFSKDSLKKIHSINKNIPLVQLLWYYPNENNEIVEWSGITHEPKRVTNDDFQEIKKY-----AVGIGPNLRNDNGDLIINESYMKMARQNGLL-IHPYTINEKPDMRLLMKWGATGMFTNYPDRLHTVLK--

General information: TITO was launched using: RESULT: Template: 4R7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1089 7318 6.72 32.38 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain G : 0.77 3D Compatibility (PKB) : 6.72 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4R7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R7O-query.scw PDB file : Tito_Scwrl_4R7O.pdb: