Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEM--DDMMSLSGIGKGTYSVIKEYIDEGKSSTLESLQKEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEACEQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALRIAREIEEHLSQFTHIIKFSRAGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYE-TSVDFRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFYAHFGLPLIPPEIRESGQE--VETYSDSIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIKKGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEFENFRILKGVEMDILPDGTLDYDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEIDIDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGVTAARKGWTETKNVLNARSLKDVEAFLKRND 2W9M Chain:B ((13-559)) -----LVHALERTADLLDILGGEDFKSRAYRSAARSLEELNEETPELLAREFTGIPKVGKGIAAELSDFARSGTFAPLEAAAGQLPPGLLDLLGVRGLGPKKIRSLWLA-GIDSLERLREAAESGELAGLKGFGAKSAATILENVVFLFEARQRQSLRAGLAVAEELAGALTDLSP----APAGDVRRGLETVRAAELTV-TGTPDDVLARLPEL------TVQGDG----VLSGDYEGVPVEIACAPAEAR-GALDLLRSGEHFAGQVQAAAQARGFTLTAGGLSRGD--EVLPTPTEAVVFHALDLPFRPAEYREPEHDDLWQTLPDPAELVTVGDLRGMIHTHSTWSDGGASIREMAEATLTLGHEFLGTADHSRAAYYANGLTIERLREQLKEIREL--QRAGLPIVAGSEVDILDDGSLDFPDDVLGELDYVVVSVHSNFTLDAARQTERLIRAVSHPLVTVLGHATGRLLLRRPGYALDLDAVLGACEANGTVVEINANAARLDLDWREALRWRER-LKFAINTDAHVPGGLRDARYGVMQARKAGLTPAHVVNSLGRAEFLDFVARQ-

General information: TITO was launched using: RESULT: Template: 2W9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3128 159005 50.83 293.37 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 50.83 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2W9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W9M-query.scw PDB file : Tito_Scwrl_2W9M.pdb: