Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPEALFPHLKQVFGIQSFSLAIKCDSRLDDIKATALKAIKDQYKPGDTFKVATKRAYKQFELDTNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATFLTIRDEKGAGGLPVGSAGKAMLMLSGGFDSPVAGFYAMK-RGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFGGSM-TLHIVPFTKTQE-L-----IQKQ----IPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLESMYAINA-VTSTPILRPLIA--MDKTEIIEKSREIGTYETSIQPFEDCCTIFTPPSPKTRPKKEKIEHFESFVDFEPYIQEAVDNIETMTLYSEQEANDKFAELF 4XFJ Chain:A ((10-177)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGG--EDMEVVRQRALDC-----GAVESIVIDARDEFANDYCVPAIQSNALYMDRYPLVSALSRPLIVKHLVKAAREHGGTIVAHGCTGKG-NDQVR-FEVGFASLAPDLEVLAPVRDYAWTREKAIAFAEENNIPIN-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4XFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 720 2535 3.52 16.78 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 3.52 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_4XFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XFJ-query.scw PDB file : Tito_Scwrl_4XFJ.pdb: