Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEQLIQAVNKQVANWTVMYVKLHNYHWYVKGKDFFTLHEKFEELYNETATYIDDLAERLLALNGKPIATMKESLETASVKEAAGNETAEQMVQSVYDDFTVIAEELKNGMDLADEVGDETTGDMLLAIHQNIEKHNWMLKAYLG 1JIG Chain:C ((6-145)) ----VVEVLNKQVANWNVLYVKLHNYHWYVTGPHFFTLHEKFEEFYNEAGTYIDELAERILALEGKPLATMKEYLATSSVNEGTSKESAEEMVQTLVNDYSALIQELKEGMEVAGEAGDATSADMLLAIHTTLEQHVWMLSAFL-

General information: TITO was launched using: RESULT: Template: 1JIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 569 -58505 -102.82 -417.89 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.93 3D Compatibility (PKB) : -102.82 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_1JIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JIG-query.scw PDB file : Tito_Scwrl_1JIG.pdb: