Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIKLEQLGYCYQKNSPFEKRALLDVNVSFDSGSYSAIIGHTGSGKSTLLQHLNALLMPTEGKITVGDREIVAGVKQKKLRDLRKKVGIVFQFPEAQLFEETVEKDICFGPMNFGVSEEDAKLRAKKVIYEVGLT-EEILSRSPFELSGGQMRRVAIAGVLAMDPEVLVLDEPTAGLDPHGREEIMEMFYNLHKEKGLTTVLVTHSMEDAARYAEKIVLMKAGTVLQIGTPREIFAKPDELVDLGLSVPDVVRFQGLFERKFDVKLTKTCLTIDELTTEMAPYLAKGGA 4HLU Chain:B ((5-232)) --IEVVNVSHIFHRGTPLEKKALENVSLVINEGECLLVAGNTGSGKSTLLQIVAGLIEPTSG-------DVLYDGERKKGYEIRRNIGIAFQYPEDQFFAERVFDEVAFAVKNFYPDRDPVPL-VKKAMEFVGLDFDSFKDRVPFFLSGGEKRRVAIASVIVHEPDILILDEPLVGLDREGKTDLLRIVEKW-KTLGKTVILISHDIETVINHVDRVVVLEKGKKVFDGTRMEFLEKYD--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4HLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -115456 for 1828 contacts (-63.2/contact) + 2D Compatibility (PS) -24789 + (NN) -12052 + (LL) 4528 1D Compatibility (HY) -16800 + (ID) 4100 Total energy: -168669.0 ( -92.27 by residue) QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_4HLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HLU-query.scw PDB file : Tito_Scwrl_4HLU.pdb: