Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MNLIRIELRKMKMGWYIRGALIANVIIMGFMWLISYSEKADGGVSFQSTDEAFLIIGTFVRAVFIVFGAVLIVKLVISEYKNKTILVMFTYPISRKKLLTAKLMIAGGLTFITILL----SNILIAAGFFW------LNSICHFIPGEL--TSEIISQQAVKMAVFAFGAAGTSLVPIFFGMRRHSVPATIISSVVIVMLISSTSPGFSISSVVYIPLSLAAFGLFFSYMAIRNADKQDA 4CTD Chain:A ((3-270)) RNDWHFNIGAMYEIENVDGLAEPSVYFNAANGPWRIALAYYQEGPVDYTWFDRPELEVHYQFLENDDFSFGLTGGFRNYGYHYVDDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFANDLADTRVETETGLQYTFNETVALRVNYYLERGFNNNGEFSTQEIRAYLPLTLGN--HSVTPYTRIGLDRAGHDFNRVGLFYGYDFQN---GLSVSLEYAFEWQDK-----FHYAGVGVNYSFHH

General information: TITO was launched using: RESULT: Template: 4CTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 993 -85170 -85.77 -390.69 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -85.77 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.64 QMean score : -0.016

(partial model without unconserved sides chains): PDB file : Tito_4CTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CTD-query.scw PDB file : Tito_Scwrl_4CTD.pdb: