Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSA-Q---PKADTQAYIQNWADQYSQYDRFLKGE-------------------NAYVAGVLYDFLEKARASNVSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAEAREKAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKNGAHEGMGTYIIQKLVKGAFGRLDFTYRHP-IFRLEIKIPFQK 4BIX Chain:A ((79-295)) ---------------------------------------------------------------------------------------------------------------------------SQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELERIETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTVNFPPGPWPLYGNPNALESALENIVRNALRYSHT-----KIEVGFAVDKDGITITVDDDGPGVSPEDREQIFR-PFYRTD---GTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIWLPLY-

General information: TITO was launched using: RESULT: Template: 4BIX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 724 -84985 -117.38 -459.38 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -117.38 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4BIX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BIX-query.scw PDB file : Tito_Scwrl_4BIX.pdb: