Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKKWSGLFVIAACFLLVAACGNSSTKGSADSKGDKLHVVTTFYPMYEFTKQIVKDKGDVDLLIPSSVEPHDWEPTPKDIANIQDADLFVYNSEYMETWVPSAEKSMGQGHAVFVNASKGIDLMEGSEEEHEEHDHGEHEHSHAMDPHVWLSPVLAQKEVKNITAQIVKQDPDNKEYYEKNSKEYIAKLQDLDKLYRTTAKKAEKKEFITQHTAFGYLAKEYGLKQVPIAGLSPDQEPSAASLAKLKTYAKEHNVKVIYFEEIASSKVADTLASEIGAKTEVLNTLEGLSKEEQDKGLGYIDIMKQNLDALKDSLLVKS 3HJT Chain:B ((14-286)) ------------------------------------MSVVTSFYPMYAMTKEVSGDLNDVRM-IQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDP---KSKVNVFEASKPLTLDRVK----------------LYDPHTWTDPVLAGEEAVNIAKELGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGLKQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKTLSPLEAAPSGNKT----YLENLRANLEVLYQQL----

General information: TITO was launched using: RESULT: Template: 3HJT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1367 -92231 -67.47 -361.69 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -67.47 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_3HJT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HJT-query.scw PDB file : Tito_Scwrl_3HJT.pdb: