Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLSKGQRIDLTKTNPGLTKAVIGLGWDTNKYSGGHDFDLDASAFLVDAHDNCVNDLDFVFYNNLEHPSGGVIHTGDNRTGEGDGDDEQIIVDFSKIPAHIEKIGITVT-IHDAEARSQNFGQVSNAFVR-VVDEETQNELLRFDLGEDFSIETAVVVCELYRHGGEWKFNAIGSGFSGGLAALCRNYGLQV 3RPF Chain:A ((3-147)) AVLKIIQGA----------LDTRELLKAYQEEACAKNFGAFCVFVGIVRKEDN--IQGLSFDIY------------------------EALLKTWFEKWHHKAKDLGVVLKMAHSLG--DVLIGQSSFLCVSMGKNRKNALELYE-NFIEDFKHNAPIWKYDLI-HNKRIYAKERSHPLKGS--------GLL-

General information: TITO was launched using: RESULT: Template: 3RPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 584 -66152 -113.27 -462.60 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -113.27 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_3RPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RPF-query.scw PDB file : Tito_Scwrl_3RPF.pdb: