Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISFSESQKLFAYFSGLIAALSLFIYYVSAQQSEGALILCITFGVIAAGIWFGPIYALAVTLIVLFVLGTLMMFFQTGQTSLFPAEEGLRMLVVWGIALLLFSFISGRIHDITAELRRSMTRLQSEIKSYVAVDRVTGFDNKQRMKLELSEEIKRAERYGNSFVFLLLHMHYFKEFKSLYGEKETDRLFQYVGQQIRTSVRETDKKFRPSDERIGIVLTHTPAEHMPAVLTKLKKQLDTYQLENGKYVSLTFHVCYLPYR---NDIQTADQFLEELENEMMMNEL 3EZU Chain:A ((174-331)) ---------------------------------------------------------------------------------------------------------------------------EARQLAAQSHFDALTGVMTRAGFFKTVGSLAYAAQRSGSNVGIMLIDLDYFKLVG-----QTGDRILQLVAETITSHLRRSDVVGRYDGDEFVVYLSPVEPASLRTVAENLRRSIEEES---ARMVPVTASIGVAQGILGTDVDGGIEELVRLADECLMQA--

General information: TITO was launched using: RESULT: Template: 3EZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 632 -98429 -155.74 -656.19 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -155.74 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_3EZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZU-query.scw PDB file : Tito_Scwrl_3EZU.pdb: