Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQIRTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF 4HKE Chain:B ((1-116)) MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQIRTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

General information: TITO was launched using: RESULT: Template: 4HKE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 546 -80313 -147.09 -692.35 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.98 3D Compatibility (PKB) : -147.09 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_4HKE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HKE-query.scw PDB file : Tito_Scwrl_4HKE.pdb: