Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLNFIVPDINMTFGDMKFMGLNRERYVYDRENNKRTDVLESRIYNIASAVQG-GQIEVTIPEYAGAKEIPPFADIELKN---PKISAMATSQRDSTYANVMWKLEADDIVVKGGSSVKPAAATGGNEKK 2HIN Chain:A ((1-66)) ----------------------------------MKPEELVRHFGDVEKAAVGVGVTPGAVYQWLQAGEIPPLRQSDIEVRTAYKLKSDFTSQRMGKEGH------------------------------

General information: TITO was launched using: RESULT: Template: 2HIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 209 -7964 -38.11 -128.45 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -38.11 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_2HIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HIN-query.scw PDB file : Tito_Scwrl_2HIN.pdb: