Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKLFHSTLIVLLFFSFFGVQPIHAKKQFKVPNS---VASISKENTYPNASQDQPMLQPSKLAKELLDHSEVKIENPHLIKMLNESNISGTPLAVGYRATIFLGKWALGYESNETVANWEYKKINTNRADNRGGKETAEMHYAQEQQYRVKGGLTAKVPNAEDVKSMMMQKAMKKTNLPLAFETVIGAGTKRDQIYKVAPKKIGYLHAYAPAVNEKGKVTYGEVYLVLKGNKRKLVVKNVTSQGIGAWIPVQDHVTFGFQLSSLPR 4D7G Chain:A ((72-123)) ----------------SFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYADSQRPISLPSKGDRNVI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4D7G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 54 -3357 -62.16 -68.50 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -62.16 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_4D7G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D7G-query.scw PDB file : Tito_Scwrl_4D7G.pdb: