Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVYITYGTADFLKTIVQKHPSENILLMQGQENAILIHETNGDTVFQAPHAYEVIDQVGEIKHPGFAVLNNIAVTQEGRPLFENRFKNRAGKVENEPGFEAIRVLRPLDSDTYVILTLWETESAFQDWQQSGSYKEAHKKRDTSAGIDTTSIFSRPSYVTTYFAVE 4FVC Chain:E ((1-166)) MKVYITYGTADFLKTIVKKHPSENILLMQGQENAILIHETSGDTVFQAPHAYEVIDQVGEIKHPGFAVLANIAVTQEGRPLFENKFKNRAGKVENEPGFEAIRVLRPLDSDTYVILTLWETERAFQDWQQSDSY---------------TSIFSRPSYVTTYFAVE

General information: TITO was launched using: RESULT: Template: 4FVC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 722 -96429 -133.56 -638.60 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain E : 0.96 3D Compatibility (PKB) : -133.56 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_4FVC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FVC-query.scw PDB file : Tito_Scwrl_4FVC.pdb: