Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKRETFNETFLKAARGEKADHTPVWYMRQAGRSQPEYRKLKEKYGLF-EITHQPELCAYVTRLPVEQYGVDAAILYKDIMTPLPSIGVDVEIKNGIGPVIDQPIRSLADIEKLGQIDPEQDVPYVL---ETIKLLVNEQLNVPLIGFSGAPFTLASYMIEGGPSKNYNKTKAFMYSMPDAWNLLMSKLADMIIVYVKAQIEAGAKAIQIFDSWVGALNQADYRTYIKPVMNRIFSELAKE----NVPLIMFGVGASHLAGDWHDLPLDVVGLDWRLGIDEARSKGITK-TVQGNLDPSILLAPWEVIEQKTKEILDQGM---ESDGFIFNLGHGVFPDVSPEVLKKLTAFVHEYSQNKKMGQYS 4ZR8 Chain:B ((14-360)) -------NDRFLRALLREPVDTTPIWMMRQAGRYLPEYRETRSKAGDFLSLCKNTEFACEVTLQPLRRYDLDAAILFSDILTIPDALGLGLYFETGEGPKFHKTVRTEQDVANLPKLNAKADLDYVMNAVSTIRSALGGQ--VPLIGFSGSPWTLATYMVEGGSSKEFRFTKQMMYAQPEVLHALLDHLADSVIDYLNAQIDAGAQAIQIFDSWGGALAHREYVEFSLNYMKKIIAGLQREKDGRRIPVIVFTKGGGQWLEPMITTGADALGLDWTTPLNTARTTVAGRVALQGNLDPAVLYGSAASIEKAVKAMLDDAYANGEKTGYVANLGHGITQWVDPAQPKIFVDTVHEYS---------

General information: TITO was launched using: RESULT: Template: 4ZR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1945 -224344 -115.34 -669.68 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -115.34 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_4ZR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZR8-query.scw PDB file : Tito_Scwrl_4ZR8.pdb: