Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQEVVLSIDAIQEPEQIKFNMSLKNQSERAIEFQFSTGQKFELVVYDSEHKERYRYSKEKMFTQAFQNLTLESGETYDFSDVWKEVPEPGTYEVKVTFKGRAENLKQVQAVQQFEVK 3NRF Chain:A ((27-83)) ----------------RTQAFKVRLVNAAKSEISLK--N---SCLVAQSAAGQS-FRLDTVDE---ELTADTLKPGASVEGD-------------------------------------

General information: TITO was launched using: RESULT: Template: 3NRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 157 -11978 -76.29 -210.14 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -76.29 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3NRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NRF-query.scw PDB file : Tito_Scwrl_3NRF.pdb: