Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQLSEDQIVKVTGDVSSPKGFQAKGVHCGLRYS-KKDLGVIISETPAVSAAVYTQSHFQAAPIKVTQDSLKHGPTLKAVIVNSAIANACTGEQGLKDAYTMRESFASQLGIEPELVAVSSTGVIGEHLDMEKIHAGIELLKETPAGSGDFEEA---ILTTDTVIKQTCYELAIGGKTVTIGGAAKGSGMIHPNMATMLGFVTTDAAIEEKALQKALREITDVSFNQITVDGETSTNDMVLVMANGCAENECLTEDHPDWPVFKKALLLTCEDLAKEIARDGEGATKLIEAQVQGAKNNLDANVIAKKIVGSNLVKTAVYGTDANWGRIIGAIGHSA--AQVTAEEVEVYLGGQCLF--KNNEPQPFSESIAKEYLEGDEITIVIKMAEGDGNGRAWGCDLTYDYIKINASYRT 1VZ7 Chain:C ((8-390)) -----------------TPRGFVVHTAPVGLADDGRDDFTVLASTA--TVSAVFTRSRFAGPSVVLCREAVADGQA-RGVVVLARNANVATGLEGEENAREVREAVARA---PEGEMLIASTGVIGRQYPMESIREHLKTL-EW-------DRAARAIMTTDTRPKEV--RV-----TLV--GIAKGVGMLEPDMATLLTFFATDARLDPAEQDRLFRRVMDRTFNAVSIDTDTSTSDTAVLFANGLA-------GEVDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVAMAIGKCSDDTDIDQERVTIRFGEVEVYPP-----DDALRAAVAEHLRGDEVVIGIDLAIADGAFTVYGCDLTEGYVRLNSE---

General information: TITO was launched using: RESULT: Template: 1VZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2102 -183557 -87.32 -522.95 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -87.32 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_1VZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VZ7-query.scw PDB file : Tito_Scwrl_1VZ7.pdb: