Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKCARLNDRIIHLHTYSREHYQFLFEEGIKGHLFCSHCGKPVLLRLNIADPPEFIHRQPGDFPACEEACEPKPSKEGKKEDDQESGVIRLPKGKAIAADPSPAVTEWHRPRSIKPGTPFVPKTIEPDTSLFPSVGLNTDQLKAVTETEGPLLVLAGAGSGKTRVLTARAAHMIEHLGIPPENMLLVTFTTKAVAEMKERMANQYGLQPAKVRRIVTGTFHSLFYKILYHSNSAKWNGEHL-------LKMEWQREQYIKKALYE-EGIDEKESP------VDQALQQIGFWKNTYVPNERIPLKDEWEKQVY-RLYEHYERQKKEHSQFDFDDMASACYELFIERPDLLEQYQSRFTYILIDEFQDINPVQYKIMQMLASPEQNLCCVGDDDQSIYAFRGSNPSFILDFQKDYPGAKTIYLTANYRSTHPIVSSADIVVKKNKNRYAKTLEAARDDIQVPVLFY-PYDEEEEATMVVSDIKEKIQNGASPEDFAVLYRTNSGGRAIYERLHQSSIPYTADRGVQSFYSRRIVRQILAYLYASQNEDDTEAI--KHLLPALFLKQSALNTLKALSITEDCTMIKALAKLPDLKPFQLDKIKKIVPFFASLR--------TMKPVEAITFAEGKMGFSEYLKKRGNEG-----------NKLEKGSDDLRDIKVVAKKFKTIPDFLAHVDHMRA-AEKNRTDEHGVQLMTIHRSKGLEFKTVYVLGTVDGSIPHDFSLETARKGDEAALEEERRLLYVAMTRAKQHLYLSCPANRR--GKT--ANRSRFLYPLLQKARQPLHH 4C2T Chain:C ((13-645)) ---------------------------------------------------------------------------------------------------------------------------------------LNPTQAQAADHFTGPALVIAGAGSGKTRTLIYRIAHLIGHYGVHPGEILAVTFTNKAAAEMRERAGH---LVPG-AGDLWMSTFHSAGVRIL------RTYGEHIGLRRGFVIYDDDDQLDIIKEVMGSIPGIGAETQPRVIRGIIDRAKSNL--WTPDDLDRSREPFISGLPRDAAAEAYRRYEVRKKGQNAIDFGDLITETVRLFKEVPGVLDKVQNKAKFIHVDEYQDTNRAQYELTRLLASRDRNLLVVGDPDQSIYKFRGADIQNILDFQKDYPDAKVYMLEHNYRSSARVLEAANKLIENNTERLDKTLKPVKEAGQ-PVTFHRATDHRAEGDYVADWLTRLHGEGRAWSEMAILYRTNAQSRVIEESLRRVQIP-RIVGGV-GFYDRREIRDILAYARLALNPADDVALRRIIGRPRRGIGDTALQKLMEWARTHHTSVLTACANAAEQNI--LDRGAHKATEFAGLMEAMSEAADNYEPAAFLRFVMETSGYLDLLRQE--EGQVRLENLEELVSAAEEWSQDEAN---SIADFLDDAALLSSVDDMRTKAE----PEDAVTLMTLHNAKGLEFPVVFIVGVEQGLLPSKGAIAEGPSG----IEEERRLFYVGITRAMERLLMTAAQNRMQFGKTNAAEDSAFL-------------

General information: TITO was launched using: RESULT: Template: 4C2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 3010 -219306 -72.86 -377.46 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -72.86 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_4C2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C2T-query.scw PDB file : Tito_Scwrl_4C2T.pdb: