Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYGIVLFPSK--KLQDLANSYRKRYD---P-SYSLIPPHLTLRASFECAEEKADQLVSHLRNIAKE--SHPLVLKMTKYSSFAPVNNVIYIKAEPTEELKTLNEKLYTGVLA--G--EQEYNFVPHVTVGQNLSDDEHS-DVLGQLKMQEV--SHEEIVDRFHLLYQLENGSWTVYETFLLGRGE 4Z5V Chain:A ((7-167)) PNHFINFPLAQFSGFMGKYLKLQSQLVEMGLDCKLQKAPHVSITLLDIKA-DQYKQVEFAIQEIIDDLAAYEGDIVFDNPHMLG----RCLV-L-DVRGFEELHEDIVEILRRRGCTADQSRHWIPHCTVAQFDEERETKGMQFYHKEPFYLKHNNLLTDAGLELVKI------------------

General information: TITO was launched using: RESULT: Template: 4Z5V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 609 -75767 -124.41 -518.95 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -124.41 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_4Z5V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z5V-query.scw PDB file : Tito_Scwrl_4Z5V.pdb: