Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQTFEG-QVACFQMDVRSDSAASDMIKEAVKAFGRLDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQAAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGVLSLTRTLAVEWGSKYGIRTNAIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDCITMDGGQWLNPYPF 1W6U Chain:C ((47-270)) -----------------------SSLGAQCVIASRKMDVLKATAEQISSQTGNKVHAIQCDVRDPDMVQNTVSELIKVAGHPNIVINNAAGNFISPTERLSPNAWKTITDIVLNGTAFVTLEIGKQLIKAQKGAAFLSITTIYAETGSGFVVPSASAKAGVEAMSKSLAAEWG-KYGMRFNVIQPGPIKT-GAF------GTFEKEMIGRIPCGRLGTVEELANLAAFLCSDYASWINGAVIKFDGGE-------

General information: TITO was launched using: RESULT: Template: 1W6U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 967 -99045 -102.42 -458.54 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -102.42 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_1W6U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W6U-query.scw PDB file : Tito_Scwrl_1W6U.pdb: